Molecular detection of cholera toxin genes in Vibrio cholerae infection in human

Cholera is an intestinal infection caused by Vibrio cholerae. The hallmark of the disease is profuse secretory diarrhea. Cholera can be endemic, epidemic, or pandemic. A total of 15 Vibrio cholerae isolates were obtained from diarrheal samples by standard bacteriological and serological methods. After DNA extraction, a polymerase chain reaction (PCR) assay was performed for detection of the presence of the virulence associated gene such as ctxAB, hly, ace and tcp genes. In the present study, it was seen that hly gene was found in all isolates understudy, whereas tcp was detected in 13 isolates of V. cholerae. Moreover, ten isolates were shown to possess ace gene in their genome. On the other hand only seven isolates V. cholerae were shown to possess ctxAB gene. Sequencing of the tcp gene from the isolates revealed a novel mutation in the gene. Whereas no mutation found in ctxAB and ace genes. Vibrio cholerae genome sequence provides a new starting point for the study of this organism's environmental and pathobiological characteristics. It may also provide important clues to understand the metabolic and regulatory networks that link genes on the chromosomes.